The association of -799C/T and -381A/G matrix metalloproteinase 8 (MMP-8) gene polymorphisms with periodontitis and active MMP-8 levels in the oral cavity

Fragkioudakis Ioannis^a, Ntolkeras Antonis^b, Kottaridi Christina^c, Grigoriadis Andreas^d, Sakellari Dimitra^a

^aAristotle University of Thessaloniki,School of Dentistry, Department of Periodontology and Implant Biology, Thessaloniki, Greece
^bAristotle University of Thessaloniki, School of Biology, Development and Molecular Biology, Department of Genetics,General Microbiology Laboratory, Thessaloniki, Greece
^cAristotle University of Thessaloniki, School of Biology, Department of Genetics, Development and Molecular Biology General Microbiology Laboratory, Thessaloniki, Greece
^dGeneral Military Training Hospital, Department of Periodontology, Thessaloniki, Greece

Abstract

Background/Aim: To investigate the relationship of the -799C/T (rs11225395) and -381A/G (rs1320632) matrix metalloproteinase 8 (MMP8) gene polymorphisms with periodontitis and the levels of active MMP-8 (aMMP-8) in an oral rinse. Material and Methods: Eighty subjects that had participated in a previous study contributed with an oral rinse sample collected before full-mouth periodontal clinical assessments. In addition, aMMP-8 levels in an oral rinse were quantified with a chairside point of care (PoC) test (PerioSafe), and the accompanying digital reader (OraLyzer). After DNA extraction, the samples were amplified with Polymerase Chain Reaction using specific primers. The amplified DNA samples were analyzed with a sequencing assay. Results: All of the patients were homozygous for the -381A/A genotype while 21.2% of the subjects were homozygous for the -799T/T genotype. No significant association was found between the -799T/T and periodontal disease, however, the presence of the specific genotype was significantly associated with the stage of periodontitis and the levels of aMMP-8 in the oral cavity. Conclusions: The results of the current study of 80 Greek subjects indicated that the presence of the rs11225395 (-799 T/T) genotype may be correlated with the severity of the periodontal disease. In addition, patients homozygous for the T/T genotype had higher levels of aMMP-8.

Keywords: periodontitis; polymorphisms; MMP8

Reference

Fragkioudakis I, Riggio MP, Apatzidou DA. Understanding the microbial components of periodontal diseases and periodontal treatment-induced microbiological shifts. J Med Microbiol, 2021;70. doi: 10.1099/jmm.0.001247

Papapanou PN, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH, Flemmig TF, et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol, 2018;89:S173-S182

Graziani F, Tsakos G. Patient-based outcomes and quality of life. Periodontol 2000, 2020;83:277-294

Luchian I, Goriuc A, Sandu D, Covasa M. The Role of Matrix Metalloproteinases (MMP-8, MMP-9, MMP-13) in Periodontal and Peri-Implant Pathological Processes. Int J Mol Sci, 2022;23:1806

Gupta N, Gupta ND, Gupta A, Khan S, Bansal N. Role of salivary matrix metalloproteinase-8 (MMP-8) in chronic periodontitis diagnosis. Front Med, 2015;9:72-76

Kraft-Neumärker M, Lorenz K, Koch R, Hoffmann T, Mäntylä P, Sorsa T, Netuschil L. Full-mouth profile of active MMP-8 in periodontitis patients. J Periodontal Res, 2012;47:121-128

Zhang L, Li X, Yan H, Huang L. Salivary matrix metalloproteinase (MMP)-8 as a biomarker for periodontitis: A PRISMA-compliant systematic review and meta-analysis. Medicine (Baltimore), 2018;97:e9642

Birkedal-Hansen H. Role of matrix metalloproteinases in human periodontal diseases. J Periodontol, 1993;64:474-484

Konopka L, Pietrzak A, Brzezińska-Błaszczyk E. Effect of scaling and root planing on interleukin-1b, interleukin-8 and MMP-8 levels in gingival crevicular fluid from chronic periodontitis patients. J Periodontal Res, 2012;47:681-688

Alassiri S, Parnanen P, Rathnayake N, Johannsen G, Heikkinen AM, Lazzara R, et al. The Ability of Quantitative, Specific, and Sensitive Point-of-Care/ Chair-Side Oral Fluid Immunotests for aMMP-8 to Detect Periodontal and Peri-Implant Diseases. Dis Markers, 2018;2018:1306396

Chou YH, Ho YP, Lin YC, Hu KF, Yang YH, Ho KY, et al. MMP-8-799 C>T genetic polymorphism is associated with the susceptibility to chronic and aggressive periodontitis in Taiwanese. J Clin Periodontol, 2011;38:1078-1084

Emingil G, Han B, Gürkan A, Berdeli A, Tervahartiala T, Salo T, et al. Matrix Metalloproteinase (MMP)-8 and Tissue Inhibitor of MMP-1 (TIMP-1) Gene Polymorphisms in Generalized Aggressive Periodontitis: Gingival Crevicular Fluid MMP-8 and TIMP-1 Levels and Outcome of Periodontal Therapy. J Periodontol, 2014;85:1070-1080

Izakovicova Holla L, Hrdlickova B, Vokurka J, Fassmann A. Matrix metalloproteinase 8 (MMP8) gene polymorphisms in chronic periodontitis. Arch Oral Biol, 2012;57:188-196

Murthykumar K, Varghese S, Priyadharsini JV. Association of MMP8 (-799C/T) (rs11225395) gene polymorphism and chronic periodontitis. Drug Invention Today, 2019;11:1594-1597

Grigoriadis A, Koutounidou S, Räisänen I, Arsenakis M, Sakellari D. Interaction between TCF7L2 rs7903146 Genotype, HbA1c Levels, and the Periodontal Status of Dental Patients. Eur J Dent, 2021;15:495-501

Görgülü NG, Doğan B. Effect of non-surgical periodontal treatment on salivary and serum biomarkers in Stage III Grade B and C periodontitis. J Periodontol, 2022;93:1191-1205

Weng H, Yan Y, Jin YH, Meng XY, Mo YY, Zeng XT. Matrix metalloproteinase gene polymorphisms and periodontitis susceptibility: a meta-analysis involving 6,162 individuals. Sci Rep, 2016;6:24812

Balkan Journal of Dental Medicine, 2023, vol. 27, br. 3, str. 176-180

Fragkioudakis Ioannisa, Ntolkeras Antonisb, Kottaridi Christinac, Grigoriadis Andreasd, Sakellari Dimitraa

Abstract

Reference

Fragkioudakis Ioannis^a, Ntolkeras Antonis^b, Kottaridi Christina^c, Grigoriadis Andreas^d, Sakellari Dimitra^a