Oliver Dimitrovski1,2, Vancho Spirov1, Blagoja Dastevski3, Filip Koneski2
1Department of Oral Surgery and Implantology, University Dental Clinical Center “St. Pantelejmon”, Skopje, FYR Macedonia
2Department of Oral Surgery, Faculty of Dental Medicine, Ss. Cyril and Methodius University in Skopje, FYR Macedonia
3Department of Prosthodontics, Faculty of Dental Medicine, Ss. Cyril and Methodius University in Skopje, FYR Macedonia
Summary
Background/Aim: Cysts which appear in the orofacial region are represented as common pathological changes which underlying mechanism of development is still not fully clear. In recent years, a dominant role in the pathogenesis of cysts belongs to the immunopathological reactions. It is assumed that the loss of bone in cysts is due to the presence of complementary cascades, prostaglandins synthesis and numerous neutrophil granulocytes. The main objective was to determine the levels of Ig G, A and M in serum and saliva of patients with radicular, residual and periodontal cysts before and after the surgical treatment.
Material and Methods: The study included 185 patients, of which 150 patients were diagnosed with inflammatory cysts (radicular, periodontal and residual), while the control group consisted of 35 patients without presence of inflammatory cyst. The immunoglobulins were determined prior to the surgical removal of the cyst and one month after the procedure, when complete clinical wound healing was observed. The levels of these immunological markers were compared to each other before and after the cyst extirpation, taking into consideration the different cyst types. A comparison was also made between both examination and control group.
Results: The difference of the basic values of the immunoglobulins before therapy and the basic values of the immunoglobulins in the control group was statistically significant only in the group of residual and periodontal cysts for IgG and IgM The difference of the average values of immunoglobulins (IgG, IgA and IgM) in the group with residual cysts before and one month after therapy is statistically significant (p=0.0000; p=0.0371; p=0.0276). A significant difference was registered in IgA among the three examined groups one month after surgical intervention.
Conclusions: The levels of serum immunoglobulins in patients with inflammatory cysts were elevated before the treatment and dropped after the cyst removal. This study suggests that the IgA, IgG and IgM may play an important role in the occurrence, development and persistence of the cystic lesions.
1. Piattelli A, Rubini C, Iezzi G, Fioroni M. CD1a-positive cells in odontogenic cysts. J Endod, 2002;28:267-268.Google Scholar
2. Bernardi L, Visioli F, Nör C, Rados PV. Radicular Cyst: An Update of the Biological Factors Related to Lining Epithelium. J Endod, 2015;41:1951-1961.Google Scholar
3. Aminoshariae A, Kulild JC, Mickel A, Fouad AF. Association between Systemic Diseases and Endodontic Outcome: A Systematic Review. J Endod, 2017;43:514-519Google Scholar
4. Tay JY, Bay BH, Yeo JF, Harris M, Meghji S, Dheen ST. Identification of RANKL in osteolytic lesions of the facial skeleton. J Dent Res, 2004;83:349-353.Google Scholar
5. Gomes MS, Blattner TC, Sant’Ana Filho M, Grecca FS, Hugo FN, Fouad AF, et al. Can Apical Periodontitis Modify Systemic Levels of Inflammatory Markers? A Systematic Review and Meta-analysis. J Endod, 2013;39:1205-1217.Google Scholar
6. Graves DT, Cochran D. The contribution of interleukin-1 and tumor necrosis factor to periodontal tissue destruction. J Periodontol, 2003;74:391-401.Google Scholar
7. Márton IJ, Kiss C. Overlapping protective and destructive regulatory pathways in apical periodontitis. J Endod, 2014;40:155-163.Google Scholar
8. Márton IJ, Kiss C. Protective and destructive immune reactions in apical periodontitis. Oral Microbiol Immunol, 2000;15:139-150.Google Scholar
9. Meyle J, Dommisch H, Groeger S, Giacaman RA, Costalonga M, Herzberg M. The innate host response in caries and periodontitis. J Clin Periodontol, 2017;44:1215-1225.Google Scholar
10. Silva N, Abusleme L, Bravo D, Dutzan N, Garcia-Sesnich J, Vernal R, et al. Host response mechanisms in periodontal diseases. J Appl Oral Sci, 2015;23:329-355.Google Scholar
11. Piattelli A, Fioroni M, Santinelli A, Rubini C. P53 protein expression in odontogenic cysts. J Endod, 2001;27:459-461.Google Scholar
12. Rook GA, Cameron CH. An inexpensive, portable, battery-operated photometer for the reading of ELISA tests in microtitration plates. J Immunol Methods, 1981;40:109-114.Google Scholar
13. Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin. G. Immunochemistry, 1971;8:871-874Google Scholar
14. Takeda Y, Oikawa Y, Furuya I, Satoh M, Yamamoto H. Mucous and ciliated cell metaplasia in epithelial linings of odontogenic inflammatory and developmental cysts. J Oral Sci, 2005;47:77-81.Google Scholar
15. Kubota Y, Ninomiya T, Oka S, Takenoshita Y, Shirasuna K. Interleukin-1alpha-dependent regulation of matrix metalloproteinase-9 (MMP-9) secretion and activation in the epithelial cells of odontogenic jaw cysts. J Dent Res, 2000;79:1423-1430.Google Scholar
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