Dikilitaş Ahua, Karaaslan Fatiha, Seçkin Ertuğrul Abdullahb
aUsak University, Faculty of Dentistry, Department of Periodontology, Usak, Turkey
bIzmir Katip Celebi University, Faculty of Dentistry, Department of Periodontology, İzmir, Turkey
Summary
Background/Aim: Periodontal diseases are inflammatory diseases that occur against microbial pathogens. Cytokines are biologically active molecules involved in this inflammatory process. This study aims to evaluate interleukin-1 beta (IL-1b) and interleukin-6 (IL-6) cytokine levels in the gingival crevicular fluid (GCF) of individuals with stage III grade C (SIII-GC) periodontitis, gingivitis (G) and periodontally healthy (PH). Material and Methods: A total of 64 individuals, including 22 PH, 22 G and 20 SIII-GC periodontitis were included in this study. Plaque index (PI), gingival index (GI), bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment loss (CAL) parameters were evaluated. GCF samples were analyzed by enzyme-linked immunosorbent assay (ELISA) kits. Results: IL-1b and IL-6 levels in the GCF were significantly higher in the SIII-GC periodontitis group compared to the other groups (P <0.05). There was no significant difference between IL-1b and IL-6 levels in the PH and G groups (P > 0.05). GCF IL-1b and IL-6 levels were positively associated with the whole mouth and sampling area clinical periodontal parameters (P < 0.001). Conclusions: GCF IL-1b and IL-6 total amounts are effective in determining the regions and individuals under risk in SIII-GC periodontitis. Moreover, GCF IL-1b and IL-6 levels were seen to be effective determinants in differentiating gingivitis and periodontitis.
Keywords: gingival crevicular fluid; Interleukins; Interleukin-6; macrophages; periodontitis
Reference
Kinane DF, Stathopoulou PG, Papapanou PN. Periodontal diseases. Nat Rev Dis Primersm, 2017;3:17038
Graves D. Cytokines that promote periodontal tissue destruction. J Periodontol, 2008;79:1585-1591
Matsuki Y, Yamamoto T, Hara K. Interleukin-1 mRNAexpressing macrophages in human chronically inflamed gingival tissues. Am J Pathol, 1991;138:1299-1305
Schett, G, Dayer JM, Manger, B. Interleukin-1 function and role in rheumatic disease. Nat Rev Immunol, 2016;12:14-24
Kobayashi M, Squires G, Mousa A, Tanzer M, Zukor DJ, Antoniou J et al. Role of interleukin-1 and tumor necrosis factor alpha in matrix degradation of human osteoarthritic cartilage. Arthritis Rheum, 2005;52:128-135
Nakashyan V, Tipton DA, Karydis A, Livada R, Stein, S. H. Effect of 1,25(OH) 2 D3 and 20(OH)D3 on interleukin1beta-stimulated interleukin-6 and -8 production by human gingival fibroblasts. J Periodontal Res, 2017;52:832-841
Engebretson SP, Grbic JT, Singer R, Lamster IB. GCF IL-1beta profiles in periodontal disease. J Clin Periodontol, 2002;29:48-53
Kishimoto T. Interleukin-6: discovery of a pleiotropic cytocine. Arthritis Res Ther, 2006;8:S2
Yoshizaki K, Nishimoto N, Mihara M, Kishimoto T. Therapy of rheumatoid arthritis by blocking IL-6 signal transduction with a humanized anti-IL-6 receptor antibody. Spring Semin Immunopathol, 1998;20:247-259
Tanaka T, Narazaki M, Kishimoto T. IL-6 in inflammation, immunity and disease. Cold Spring Harb Perspect Biol, 2014;6:a016295
Moreira PR, Lima PM, Sathler KO, Imanishi SAW, Costa JE, Gomes RS et al. Interleukin-6 expression and gene polymorphism are associated with severity of periodontal disease in a sample of Brazilian individuals. Clin Exp Immunol, 2007;148:119-126
Mogi M, Otogoto J, Ota N, Inagaki H, Minami M, Kojima K. Interleukin 1 beta, interleukin 6, beta 2-microglobulin, and transforming growth factoralpha in gingival crevicular fluid from human periodontal disease. Arch Oral Biol, 1999;44:535-539
Loe H, Silness J. Perıodontal dısease ın pregnancy. I. Prevalence and severıty. Acta Odontol Scand, 1963;21:533-551
Silness J, Loe H. Perıodontal dısease ın pregnancy. II. Correlatıon between oral hygiene and perıodontal condtıon. Acta Odontol Scand, 1964;22:121-135
Ainamo J, Bay I. Problems and proposals for recording gingivitis and plaque. Int Dent J, 1975;25:229-235
Papapanou P, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Clin Periodontol, 2018;45:162-170
Chapple I, Mealey B, Van Dyke T, Bartold PM, Dommisch H, Eickholz P, et al. Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and PeriImplant Diseases and Conditions. J Clin Periodontol, 2018;45:68-77
Tonetti MS, Greenwell H, Kornman KS. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. J Periodontol, 2018;8:159-172
Becerik S, Öztürk VÖ, Atmaca H, Atilla G, Emingil G. Gingival crevicular fluid and plasma acute-phase cytokine levels in different periodontal diseases. J Periodontol, 2012;83:1304-1313
Toyman U, Tüter G, Kurtiş B, Bozkurt Ş, Yücel AA, Serdar M. Evaluation of gingival crevicular fluid levels of tissue plasminogen activator, plasminogen activator inhibitor 2, matrix metalloproteinase-3 and interleukin 1-b in patients with different periodontal diseases. J Periodontal Res, 2015;50:44-51
Cekici A, Kantarci A, Hasturk H, Van Dyke T. Inflammatory and immune pathways in the pathogenesis of periodontal disease. Periodontol 2000, 2013;64:57-80
Takahashi K, Takashiba S, Nagai A, Takigawa M, Myoukai F, Kurihara H, et al. 1994. Assessment of interleukin-6 in the pathogenesis of periodontal disease. J Periodontol, 65:147-153
Dalrymple SA, Slattery R, Aud DM, Krishna M, Lucian LA, Murray R. Interleukin-6 is required for a protective immune response to systemic Escherichia coli infection. Infect Immun, 1996;64:3231-3235
Cooke GS, Hill AV. Genetics of susceptibility to human infectious disease. Nat Rev Genet, 2001;2:967-977
Hughes FJ, Turner W, Belibasakis G, Martuscelli G. Effects of growth factors and cytokines on osteoblast differentiation. Periodontology 2000, 2006;41:48-72
Moreira PR, Lima PM, Sathler KO, et al. Interleukin-6 expression and gene polymorphism are associated with severity of periodontal disease in a sample of Brazilian individuals. Clin Exp Immunol, 2007;148:119-126
Shao M, Huang P, Chen R, Hu T. Interleukin-6 polymorphisms modify the risk of periodontitis: a systematic review and meta-analysis. J Zhejiang Univ Sci B, 2009;10:920-927
Reis C, DA Costa AV, Guimarães JT, Tuna D, Braga Ac Pacheco JJ et al. Clinical improvement following therapy for periodontitis: Association with a decrease in IL-1 and IL-6. Exp Ther Med, 2014;8:323-327
Lamster IB, Oshrain RL, Fiorello LA, Celenti RS, Gordon JM. A comparison of 4 methods of data presentation for lysosomal enzyme activity in gingival crevicular fluid. J Clin Periodontol, 1988;15:347-352
Özdemir H, Develioğlu H, Yamalık N, Aydın H, Eren K. Comparative analysis of the potential effect of phase I therapy on gingival crevicular fluid myeloperoxidase levels in non-diabetic and diabetic patients with periodontitis. Cumhuriyet Dent J, 2014;17:256-266
Barros SP, Williams R, Offenbacher S, Morelli T. Gingival crevicular fluid as a source of biomarkers for periodontitis. Periodontol 2000, 2016;70:53-64
Balkan Journal of Dental Medicine, Online First