Kukey Pelina, Turkmen Cafera, Cimilli Zuhre Haleb
aMarmara University, Faculty of Dentistry, Department of Restorative Dentistry, Istanbul, Turkey
bMarmara University, Faculty of Dentistry, Department of Endodontics, Istanbul, Turkey
Abstract
Background/Aim: The principal purpose of this study was to investigate the efficacy of different active ingredients to inhibit demineralization around the margins of cervical cavities in natural teeth by scanning electron microscopy and energy dispersive X-Ray elemantal analysis (SEM-EDX). Material and Methods: Thirty-two sound human molars were used. Box-shaped cavities were prepared along the cemento enamel junction (CEJ). The samples were were immersed in a demineralization solution (pH=1) maintained for 72 hours and randomly divided into 4 groups. Surfaces according to the groups were treated with potassium nitrate, arginine and calciumsodiumphosphosilicate containing remineralization agents for 14 days, respectively and the samples in control group were submitted to toothbrushing with deionized water. The samples were analyzed by using SEM-EDX analysis. Data was statistically analyzed using by one way ANOVA (analysis of variance) test and LSD (least significant difference) test for comparison between means at a significance level of 0.05. Results: SEM-EDX elemental mapping was used to evaluate the degradation from depth profiles of fluoride (F), Calcium (Ca), and phosphate (P) leaching. Micromorphological and elemental analyses were done using SEM and EDX. SEM EDX Analysis on enamel showed a significant difference between the groups except the control group (p<0.05). The dentine results showed significant differences between the control group and all other groups (p<0.05). Elemental analysis showed significant differences in Ca weight percentage among the first and second observation levels in all groups (p<0.05). Conclusions: In conclusion, all tested toothpastes showed some ability to resist demineralization at the margins. The groups except control group showed better outcomes compared with the other tested samples.
Keywords: remineralization; in vitro; elemental mapping; SEM/ EDX
Reference
Alencar, C.D.M., Pedrinha, V.F., Araújo, J.L.N., Esteves, R.A., da Silveira, A.D.S., Silva, C.M. (2017) Effect of 10% strontium chloride and 5% potassium nitrate with fluoride on bleached bovine enamel. Open Dentistry Journal, 11(1): 476-484
Arifa, M.K., Ephraim, R., Rajamani, T. (2019) Recent advances in dental hard tissue remineralization: A review of literature. J Clin Pediatr Dent, 12 (2): 139-144
Asokan, S., Geethapriya, P.R., Vijayasankari, V. (2019) Effect of nonfluoridated remineralizing agents on initial enamel carious lesions: A systematic review. Indian J Dent Res, March-April 2019;30 (2): 282-290
Bae, J.H., Kim, Y.K., Myung, S.K. (2015) Desensitizing toothpaste versus placebo for dentin hypersensitivity: A systematic review and meta-analysis. Journal of Clinical Periodontology, 42(2): 131-141
Bhowmik, E., Pawar, C.D., Sharma, H.M. (2021) Comparative evaluation of fluorinol and calcium sodium phosphosilicate-containing toothpastes in the treatment of dentin hypersensitivity. International Journal of Dental Hygiene, 19(4): 421-428
Burwell, A.K., Litkowski, L.J., Greenspan, D.C. (2009) Calcium sodium phosphosilicate (NovaMin): Remineralization potential. Adv Dent Res, 21(1): 35-39
Carda-Diéguez, M., Moazzez, R., Mira, A. (2022) Functional changes in the oral microbiome after use of fluoride and arginine containing dentifrices: A metagenomic and metatranscriptomic study. Microbiome, 10(1): 159-159
Cheng, X., Xu, P., Zhou, X., Deng, M., Cheng, L., Li, M., Li, Y., Xu, X. (2015) Arginine promotes fluoride uptake into artificial carious lesionsin vitro. Australian Dental Journal, 60(1): 104-111
Fernando, D., Attik, N., Pradelle-Plasse, N., Jackson, P., Grosgogeat, B., Colon, P. (2017) Bioactive glass for dentin remineralization: A systematic review. Eng C Mater Biol Appl, 76: 1369-1377
Freda, N.M., Veitz-Keenan, A. (2016) Calcium sodium phosphosilicate had some benefit on dentine hypersensitivity. Evidence-Based Dentistry, 17(1): 12-13
Hegde, M.N., Moany, A. (2012) Remineralization of enamel subsurface lesions with casein phosphopeptide-amorphous calcium phosphate: A quantitative energy dispersive X-ray analysis using scanning electron microscopy: An in vitro study. Conserv Dent, 15 (1): 61-67
Kargul, B., Özcan, M., Peker, S., Nakamoto, T., Simmons, W.B., Falster, A.U. (2012) Evaluation of human enamel surfaces treated with theobromine: A pilot study. Oral Health Prev Dent, 10 (3): 275-282
Premnath, P., John, J., Manchery, N., Subbiah, G.K., Nagappan, N., Subramani, P. (2019) Effectiveness of theobromine on enamel remineralization: A comparative in-vitro study. Cureus, 11 (9): E5686
Qu, Y., Gu, T., Du, Q., Shao, C., Wang, J., Jin, B., et al. (2020) Polydopamine promotes interfacial control. ACS Biomater Sci Eng, 6 (6): 3327-3334
2
Sforcin, J.M. (2016) Biological properties and therapeutic applications of propolis. Phytother Res, 30 (6): 894-905
Tulumbacı, F., Oba, A.A. (2019) Efficacy of different remineralization agents on treating incipient enamel lesions of primary and permanent teeth. J Conserv Dent, 22 (3): 281-286
Zawaideh, F.I., Owais, A.I., Mushtaha, S. (2017) Effect of CPP-ACP or a Potassium Nitrate Sodium fluoride dentifrice on enamel erosion prevention. J Clin Pediatr Dent, 41 (2): 135-140
Zhu, M., Li, J., Chen, B., Mei, L., Yao, L., Tian, J., Li, H. (2015) The effect of Calcium sodium phosphosilicate on dentin hypersensitivity: A systematic review and meta-analysis. PLoS One, 10 (11): e0140176
Balkan Journal of Dental Medicine, 2024, vol. 28, br. 1, str. 38-49