In Vitro Biocompatibility of Nanostructured Endodontic Materials Using SCAP Cells

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Bojana Ćetenović1 / Božana Čolović1 / Saša Vasilijić2 / Snežana Pašalić1 / Vukoman Jokanović1 / Dejan Marković3

1 Vinca Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia
2 Military Medical Academy, Faculty of Medicine, University of Defense, Belgrade, Serbia
3 School of Dentistry, University of Belgrade, Belgrade, Serbia


Background/Aim: Lately, fully innovative sol-gel method with high-temperature self-propagating reaction was used for the synthesis of new nanostructured endodontic materials, in combination with different radiopacifiers: bismuth (ALBO-MPCA1) and barium (ALBO-MPCA2). The aim of this study was to investigate the biocompatibility of nanostructured endodontic materials based on highly active calcium silicates and mixed with different radiopacifiers in comparison to MTA+ using human stem cells from the apical papilla- SCAP cells. Material and Methods: Morphology of the samples was studied by SEM. The tested materials were mixed with distilled water in a ratio 2:1 (m/m). Fifteen minutes fter the preparation, samples were used in the experiment. The biocompatibility of fresh materials, after 3h and 7 days, was tested using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide- MTT test. Results: Samples mostly consisted of spherical and rode-like. The relative viability of cells increased following the exposure time. Conclusion: The biocompatibility of synthesized materials is comparable to the control material MTA+, and therefore these materials can be recommended for for further clinical stuadies.

Keywords: Biocompatibility; Calcium Silicates; MTT; MTA; Biomaterials


  1. Wataha JC. Predicting clinical biological responses to dental materials. Dent Materi, 2012;28:23-40.Web of ScienceGoogle Scholar
  2. Camilleri J, Montesin FE, Brady K, Sweeney R, Curtis RV, Pitt Ford TR. The constitution of mineral trioxide aggregate. Dent Mater, 2005;21:297-303.CrossrefGoogle Scholar
  3. Dammaschke T, Gerth HUV, Zuchner H, Schafer E. Chemical and physical surface and bulk material characterization of white ProRoot MTA and two Portland cements. Dent Mater, 2005;21:731-738.CrossrefGoogle Scholar
  4. Sarkar NK, Caidedo R, Tirwik P, Moiseyeva R, Kawashima I. Physicochemical basis of the biologic properties of mineral trioxide aggregate. J Endod, 2005;31:97-100.Google Scholar
  5. Fridland M, Rosado R. MTA solubility: a long term study. J Endod, 2005;31:376-379.Google Scholar
  6. De Vasconcelos BC, Bernardes RA, Luna Cruz SM, Duarte MAH, De Magalhaes Padilha P, Bernardineli N, et al. Evaluation of pH and calcium ion release of new rootend filling materials. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2009;108:135-139.Google Scholar
  7. Belio-Reyes IA, Bucio L, Cruz-Chavez E. Phase composition of ProRoot mineral trioxide aggregate by X-ray powder diffraction. J Endod, 2009;35:875-878.Web of ScienceGoogle Scholar
  8. Asgary S, Parirokh M, Egbbal MJ, Brink F. Chemical differences between white and gray mineral trioxide aggregate. J Endod, 2005;31:101-103.Google Scholar
  9. Camilleri, J. Hydration mechanisms of mineral trioxide aggregate. Int Endod J, 2007;40:462-470.CrossrefWeb of ScienceGoogle Scholar
  10. Jokanovic V, Colovic B, Mitric M, Markovic D, Cetenovic B. Synthesis and properties of a new endodontic material based on nanostructured highly active calcium-silicates and calcium carbonates. Int J Appl Ceram Techol, 2014;11:57-64.Google Scholar
  11. Xu JL, Khor KA, Sui JJ, Zhang JH, Chen WN. Protein expression profiles in osteoblasts in response to differentially shaped hydroxyapatite nanoparticles. Biomater, 2009;30:5385-5391.Google Scholar
  12. Maeno S, Niki Y, Matsumoto H, Morioka H, Yatabe T, Funayama A et al. The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture. Biomater, 2005;26:4847-4855.Google Scholar
  13. International Standards Organization. Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. ISO 10993-5, 3st ed. 2009-06-01.Google Scholar
  14. Cetenovic B, Prokic B, Vasilijic S, Dojcinovic B, Magic M, Jokanovic V et al. Biocompatibility investigation of new endodontic materials based on nanosynthesized calcium silicates combined with different radiopacifiers. J Endod, 2017;43:425-432.Web of ScienceGoogle Scholar
  15. Chen C, Hsieh SC, Teng NC, Kao CK, Lee SY, Lin CK et al. Radiopacity and cytotoxicity of Portland cement containing zirconia doped bismuth oxide radiopacifiers. J Endod, 2014;40:251-254.Web of ScienceGoogle Scholar
  16. Kim EC, Lee BC, Chang HS, Lee W, Hong CU, Min KS. Evaluation of the radiopacity and cytotoxicity of Portland cements containing bismuth oxide. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2008;105:e54-e57.Google Scholar
  17. Gomes Cornélio AL, Salles LP, da Paz MC, Cirelli JA, Guerreiro-Tanomaru JM, Filho MT. Cytotoxicity of Portland cement with different radiopacifying agents: A cell death study. J Endod, 2011;37:203-210.Web of ScienceGoogle Scholar
  18. Antonijevic D, Jeschke A, Colovic B, Milovanovic P, Jevremovic D, Kisic D, et al. Addition of a Fluoridecontaining Radiopacifier Improves Micromechanical and Biological Characteristics of Modified Calcium Silicate Cements. J Endod, 2015;41:2050-2057.Google Scholar
  19. Bosso-Martelo R, Guerreiro-Tanomaru JM, Viapiana R, Berbert FL, Duarte MA, Tanomaru-Filho M. Physicochemical properties of calcium silicate cements associated with microparticulate and nanoparticulate radiopacifiers. Clin Oral Investig, 2016;20:83-90.Google Scholar
  20. Margunato S, Taşli PN, Aydin S, Karapınar Kazandağ M, Şahin F. In Vitro Evaluation of ProRoot MTA, Biodentine, and MM-MTA on Human Alveolar Bone Marrow Stem Cells in Terms of Biocompatibility and Mineralization. J Endod, 2015;41:1646-1652.Web of ScienceGoogle Scholar
  21. Petrović V, Opačić-Galić V, Živković S, Nikolić B, Danilović V, Miletić V et al. Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. Int Endod J, 2015;48:966-975.Web of ScienceGoogle Scholar
  22. Modareszadeh MR, Chogle SA, Mickel AK, Jin G, Kowsar H, Salamat N, Shaikh S, Qutbudin S. Cytotoxicity of set polymer nanocomposite resin root-end filling materials. Int Endod J, 2011;44:154-161.Web of ScienceCrossrefGoogle Scholar
  23. Wang JD, Hume WR. Diffusion of hydrogen ion and hydroxyl ion from various sources through dentine. Int Endod J, 1988;21:17-26.CrossrefGoogle Scholar

Citation Information:Balkan Journal of Dental Medicine, ISSN (Online) 2335-0245, DOI: Citation