In Vitro Biocompatibility of Nanostructured Endodontic Materials Using SCAP Cells

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Bojana Ćetenović1 / Božana Čolović1 / Saša Vasilijić2 / Snežana Pašalić1 / Vukoman Jokanović1 / Dejan Marković3

1 Vinca Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia
2 Military Medical Academy, Faculty of Medicine, University of Defense, Belgrade, Serbia
3 School of Dentistry, University of Belgrade, Belgrade, Serbia

Summary

Background/Aim: Lately, fully innovative sol-gel method with high-temperature self-propagating reaction was used for the synthesis of new nanostructured endodontic materials, in combination with different radiopacifiers: bismuth (ALBO-MPCA1) and barium (ALBO-MPCA2). The aim of this study was to investigate the biocompatibility of nanostructured endodontic materials based on highly active calcium silicates and mixed with different radiopacifiers in comparison to MTA+ using human stem cells from the apical papilla- SCAP cells. Material and Methods: Morphology of the samples was studied by SEM. The tested materials were mixed with distilled water in a ratio 2:1 (m/m). Fifteen minutes fter the preparation, samples were used in the experiment. The biocompatibility of fresh materials, after 3h and 7 days, was tested using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide- MTT test. Results: Samples mostly consisted of spherical and rode-like. The relative viability of cells increased following the exposure time. Conclusion: The biocompatibility of synthesized materials is comparable to the control material MTA+, and therefore these materials can be recommended for for further clinical stuadies.

Keywords: Biocompatibility; Calcium Silicates; MTT; MTA; Biomaterials

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Citation Information:Balkan Journal of Dental Medicine, ISSN (Online) 2335-0245, DOI: https://doi.org/10.1515/bjdm-2017-0029Export Citation

 

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