Investigation of Antioxidant Capacity of Several Luting Cements Processes by HPMC Method

[btn url=”” text_color=”#ffffff” bg_color=”#81d742″ icon=”fa-file-pdf-o” icon_position=”start” size=”14″ id=”” target=”NewWindow”]Download Article[/btn]

1 / Kosovka Obradović-Đuričić2 / Vesna Medic2 / Srđan Poštić2 / Stanislava Z Gorjanović3 / Ferenc Pastor4 / Katarina Radović2

1School of Dental Medicine, Endodont. Dpt., Belgrade University, Rankeova 4, Belgrade,Serbia
2School of Dental Medicine, Prosthodont. Dpt., Belgrade University,Serbia
3Institute of General and Physical Chemistry,Serbia
4Faculty of Chemistry, University of Belgrade, Belgrade,Serbia


Background: Free radicals (FR) occur in oral cavity where lot of food was transferred to through entire life under specific saliva conditions. Many enzymes, microorganism, alcohol beverages, nicotine and other harmful or indifferent substances when in contact to oral tissues might provoke oxidation process under specific condition creating FRʼs. The similar role might have various dental materials.

Aim: Of the study was to record the level of antioxidant (AO) activity of several permanent (P) luting cements alone or combined with quercetin AO substance.

Materials/Methods: P cements were Zn-phosphate, Zn-polycarboxilate, GIC and composite resin cement. They were prepared as original prescription and their variant by 1%weight addition of quercetin. AO activity of cements was measured by HPMC test evaluated by Student t test.

Results: There were statistically significant differences among Zn-phosphate, Zn-polycarboxilate and resin dental cements (p ˃ 0,05). GIC displayed significantly higher AO values (p < 0,01) versus other three cements. There were no difference in AO capacity between sample of original P cements and their corresponding quercetin variants (p ˃ 0,05).

Conclusions: Conventional GIC displayed the most powerful AO activity among P luting cements. Addition of 1% antioxidant quercetin did not improve AO capacity of investigated cements.

Keywords: Antioxidant; Antioxidant capacity; Free radicals; Luting cement; Glass ionomer cement; Eugenol; Quercetin; Flavone


  1. Alam N, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 2013, 21, 143-152. [Web of Science]
  2. Bourbonnais R, Leech D, Paice MG. Electrochemical analysis of the interactions of laccase mediators with lignin model compounds. Biochim Biophys Acta, 1998; 1379:381-390.
  3. Costa L, Carpentieri I, Bracco P. Post electron-beam irradiation oxidation of orthopaedic Ultra-High Molecular Weight Polyethylene (UHMWPE) stabilized with vitamin E Polym Degrad Stab, 2009; 94:1542-1547.
  4. Harish PV, Sonila A, Joseph Ambica. Iatrogenic Damage to the Periodontium Caused by Fixed Prosthodontic Treatment Procedures. Open Dent J, 2015; 9:190-196.
  5. Ho KY, Won LD, Ji JE, Tae BJ, Gil LS, Bae PH et al. Preparation and characterization of quercetin-loaded silica microspheres stabilized by combined multiple emulsion and sol-gel processes. Chem Ind Chem Eng Q, 2015; 21:85-94. [Web of Science] [Crossref]
  6. Ilic DV, Jovic P. Antioxidative potential of several eugenol preparations, post. 3rd Congress of BaSS, April 1998., Sofia, Book of abst., 33.
  7. Ilic DV, Obradovic-Djuricic K, Antonijevic Dj, Todorovic T. Eugenol based temporary luting cements possess antioxidative properties. Srp Arh Celok Lek, 2014; 141:669-674.
  8. Karoussis IK, Muller S, Salvi GE, Heitz-Mayfield LJA, Bragger U, Lang NP. Association between periodontal and peri-implant conditions: a 10-year prospective study. Clin Oral Implants Res, 2004; 15:1-7.
  9. Kokubo T, Kim HM, Kawashita M. Novel bioactive materials with different mechanical properties. Biomater, 2003; 4:2161-2175. [Crossref]
  10. Michelina C, Ferdinando P, Flavia B, Simona P, Sabina M, Paola N, Severina P. Silica/quercetin sol-gel hybrids as antioxidant dental implant materials. Sci Technol Adv Mater, 2015; 16:035001. [Crossref] [Web of Science]
  11. Murakami Y, Shoji M, Hanazawa S, Tanaka S, Fujisawa S. Preventive effect of bis-eugenol, a eugenol ortho dimer, on lipopolysaccharide-stimulated nuclear factor kapp B activation and inflammatory cytokine expression in macrophages. Biochem Pharmacol, 2003; 66:1061-1066. [Crossref]
  12. Navarro M, Michiardi A, Castano O, Planell JA. Biomaterials in orthopaedics. J R Soc Interface, 2008; 5:1137-1158. [Web of Science] [Crossref]
  13. Ponte L, Ruben V, Erika M, Diego M, Kyung-Shin P. Effects of exercise intensity on cortisol, antioxidant and DNA damage in smokers and non-smokers. FASEB J, 2015; 29:675.13.
  14. Suznjevic DS, Pastor FT, Gorjanovic SZ. Polarographic study of hydrogen peroxide anodic current and its application to antioxidant activity determine. Talanta, 2011; 85:1398-1403. [Crossref]
  15. Stansbury JW. Curing dental resins and composites by photopolymerization. J Esth Dent, 2000; 12:300-308.
  16. Trumpaite-Vanagiene R, Bukelskiene V, Alekseyuniene J, Puriene A, Baltriukiene D, Rutkunas V. Cytotoxicity of coming cements – an in vitro study. Dent Mater J, 2015; 34:294-301. [Crossref]
  17. Rabolli V, Thomassen LC, Princen C, Napierska D, Gonzalez L, Kirsch-Volders M et al. Influence of size, surface area and microporosity on the in vitro cytotoxic activity of amorphous silica nanoparticles in different cell types. Nanotoxicol, 2010; 4:307-318. [Crossref]
  18. Soheili ME, Goldberg M, Stanislawski L. In vitro effects of ascorbate and Trolox on the biocompatibility of dental restorative materials. Biomater, 2003; 24:3-9 [Crossref]
Citation Information: Balkan Journal of Dental Medicine. Volume 20, Issue 3, Pages 155–159, ISSN (Online) 2335-0245, DOI:, November 2016