Antimicrobial Activity of Three Lamiaceae Essential Oils Against Common Oral Pathogens

Miloš Nikolić1 / Tatjana Marković2 / Dejan Marković3 / Jasmina Glamočlija1 / Ana Ćirić1 / Marija Smiljković1 / 4

1Institute for Biological Research “Siniša Stanković”, University of Belgrade Belgrade,Serbia
2Institute for Medicinal Plant Research “Josif Pančić”, Belgrade,Serbia
3Faculty of Dental Medicine Department of Pediatric and Preventive Dentistry, University of Belgrade, Belgrade,Serbia
4PhD, Institute for Biological Research “Siniša Stanković”, University of Belgrade Belgrade, Boulevard Despota Stefana 142, 11000 Belgrade,Serbia

Summary

Chemical composition, antimicrobial and cytotoxic activities of commercial essential oils’ samples from the aerial plant parts of H. officinalis, R. officinalis and S. officinalis were investigated. Analyses by GC-FID and GC-MS confirmed 52 oil components. The major constituent of the H. officinalis oil was cis-pinocamphone (34.4%), followed by transpinocamphone (23.3%), and β-pinene (11.3%). Analysis of R. officinalis oil revealed 1.8-cineol as a major constituent (43.8%), as well as transpinocamphone (12.5%), α-pinene (11.5%) and β-pinene (8.2%). The most dominant constituent of S. officinalis oil was cis-thujone (32.7%), in addition to camphor (17.2%), 1.8-cineol (10.1%), α-pinene (8.6%), transthujone (7.7%) and camphene (7.3%). The essential oil antimicrobial activity assay was performed by the use of microdilution method against oral Candida spp. and bacteria, the major causative agents of a number of human oral disorders; all of them were susceptible to tested concentrations of H. officinalis, R. officinalis and S. officinalis essential oils, although the oil of S. officinalis exhibited the lowest antimicrobial potential. The results obtained in this study encourage use of investigated essential oils from Lamiaceae family in development of safe natural agents for prevention and/ or alternative therapy of human oral diseases. However, a special care during development of an effective natural preparation is required.

Keywords: Oral Pathogens; Antimicrobial Activity; Essential Oils; H. officinalis; R. officinalis; S. officinalis

References

1. Sá JM, Chong JL, Wellems TE. Malaria drug resistance: new observations and developments. Essays Biochem, 2011; 51:137-160. [Web of Science] [Crossref]

2. Hammer KA, Carson CF, Riley TV. Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol, 1999; 86:985-990. [Crossref]

3. Ríos JL, Recio MC. Medicinal plants and antimicrobial activity. J Ethnopharmacol, 2005; 100:80-84. [Crossref]

4. Betts TJ. Chemical characterisation of the different types of volatile oil constituents by various solute retention ratios with the use of conventional and novel commercial gas chromatographic stationary phases. J Chromatogr A, 2005; 936:33-46. [Crossref]

5. Burt S. Essential oils: Their antibacterial properties and potential applications in foods – A review. Int J Food Microbiol, 2004; 94:223-253. [Crossref]

6. Dagli N, Dagli R, Possible use of essential oils in dentistry. J Int Oral Health, 2014; 6:1-2.

7. Paster BJ, Olsen I, Aas JA, Dewhirst FE. The breadth of bacterial diversity in the human periodontal pocket and other oral sites. Periodontol, 2000; 42:80-87. [Crossref]

8. Da Silva-Rocha WP, Lemos VLDB., Svidizisnki TIE, Milan EP., Chaves GM. Candida species distribution, genotyping and virulence factors of Candida albicans isolated from the oral cavity of kidney transplant recipients of two geographic regions of Brazil. BMC Oral Health, 2014; 15:14-20.

9. Scully C. Candida and Oral Candidosis: A Review. Crit Rev Oral Biol Med, 1994; 5:125-157.

10. Albougy HA, Naidoo S. A systematic review of the management of oral candidiasis associated with HIV/AIDS. SADJ, 2002; 57:457-466.

11. Slots, J., Feik, D., Rams, T.E., 1990. Prevalence and antimicrobial susceptibility of Enterobacteriaceae, Pseudomonadaceae and Acinetobacter in human periodontitis. Oral Microbiol Immunol, 5; 149-154. [Crossref]

12. Evlioglu G, Koray M, Ak G, Nekora-Azak A, Topcuoglu N, Sancakli E, Alpkilic E, Kulekci G. Candida Albicans and Staphylococcus Aureus in Obturators Used for Rehabilitation of Maxillary Defects, Balk J Stom, 2013; 17:65-69.

13. Şakar O, Bilhan H, Sülün T, Ispirgil ÇFE, Erturan Z, Erköse G. Denture Related Stomatitis and Candida Counts of a Rest Home Population: An Epidemiologic Pilot Study in Patients Wearing Upper Full Removable Dentures. Balk J Stom, 2008; 12:98-102.

14. Nicolas GG, Lavoie MC. Streptococcus mutans and oral streptococci in dental plaque. Can J Microbiol, 2011; 57:1-20. [Crossref]

15. Adams RP. Identification of Essential Oil Compounds by Gas Chromatography and Mass Spectrometry (fourth ed.),” Allured Publishing Corporation: Carol Stream, 2009; IL, USA.

16. Cecchini C, Silvi S, Cresci A, Piciotti A, Caprioli G, Papa F, Sagratini G, Vittori S, Maggi F. Antimicrobial efficacy of Achillea ligustica All. (Asteraceae) essential oils against reference and isolated oral microorganisms. Chem Biodivers, 2012; 9:12-24. [Web of Science] [Crossref]

17. Nikolić M, Glamočlija J, Ćirić Ana P, Tamara M, Dejan S, Tanja SM, Antimicrobial activity of ozone gas and colloidal silver against oral microorganisms. Dig J Nanomater, 2012; 7:1693-1699.

18. Douk KD, Dagher MS, Sattout JE. Antifungal activity of the essential oil of Origanum syriacum L. J Food Protect, 1995; 58:1147-1149.

19. European Committee on Antibiotic Susceptibility EUCAST Method for determination of minimal inhibitory concentration (MIC) by broth dilution of fermentative yeasts. Discussion document E. Dis. 7.1. European Society of Clinical Microbiology and Infectious Diseases, 2002; Taufkirchen, Germany.

20. Tsukatani T, Suenaga H, Shiga M, Noguchi K, Ishiyama M, Ezoe T, Matsumoto K. Comparison of the WST-8 colorimetric method and the CLSI broth microdilution method for susceptibility testing against drug-resistant bacteria. J Microbiol Methods, 2012; 90:160-166. [Web of Science] [Crossref]

21. Glamočlija J, Soković M, Vukojević J, Milenković I, Brkić D, Van G. Antifungal activity of essential oil Hyssopus officinalis L. against micopathogen Mycogone perniciosa (Mang). Zb Matice Srp za Prir Nauk, 2005; 188.

22. Fathiazad F. A review on Hyssopus officinalis L.: Composition and biological activities. African J Microbiol Res, 2011; 5:1959-1966.

23. Salma AS. Chemical and physiological studies on anise hysop (Agastache foeniculum Pursh) and hyssop (Hyssopus officinalis L) plants grown in Egypt as new spices. Bulletin of the National Research Centre, 2002; 27:25-35.

24. Nedorostova L, Kloucek P, Kokoska L, Stolcova M, Pulkrabek J. Antimicrobial properties of selected essential oils in vapour phase against foodborne bacteria. Food Control, 2009; 20:157-160. [Web of Science] [Crossref]

25. Mazzanti G, Battinelli L, Salvatore G. Antimicrobial properties of the linalol-rich essential oil of Hyssopus officinalis L. vardecumbens (Lamiaceae). Flavour Fragr J, 1998; 13:289-294.

26. Džamić AM, Soković MD, Novaković M, Jadranin M, Ristić MS, Tešević V, Marin PD. Composition, antifungal and antioxidant properties of Hyssopus officinalis L. subsp. pilifer (Pant.) Murb. essential oil and deodorized extracts. Ind Crops Prod, 2013; 51:401-407. [Web of Science] [Crossref]

27. Weckesser S, Engel K, Simon-Haarhaus B, Wittmer A, Pelz K, Schempp CM. Screening of plant extracts for antimicrobial activity against bacteria and yeasts with dermatological relevance. Phytomedicine, 2007; 14:508-516. [Web of Science] [Crossref]

28. Celiktas OY, Kocabas EEHEH, Bedir E, Sukan FV, Ozek T, Baser KHCHC. Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chem, 2007; 100:553-559. [Web of Science] [Crossref]

29. Jordán MJ, Lax V, Rota MC, Lorán S, Sotomayor JA. Effect of bioclimatic area on the essential oil composition and antibacterial activity of Rosmarinus officinalis L. Food Control, 2013; 30:463-468. [Crossref] [Web of Science]

30. Peana AT, Moretti MD, Juliano C. Chemical composition and antimicrobial action of the essential oils of Salvia desoleana and S. sclarea. Planta Med, 1999; 65:752-754. [Crossref]

31. Yildirim A, Mavi A, Oktay M, Kara AA, Algur OF, Bilaloglu V. Comparison of antioxidant and antimicrobial activities of Tilia (Tilia argentea Desf ex DC), sage (Salvia triloba L.), and Black tea (Camellia sinensis) extracts. J Agric Food Chem, 2000; 48:5030-5034. [Crossref]

32. Soković M, Glamočlija J, Marin PD, Brkić D, van Griensven LJLD. Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model. Molecules, 2010; 15:7532-7546. [Crossref]

33. Bozin B, Mimica-Dukic N, Samojlik I, Jovin E. Antimicrobial and antioxidant properties of Rosemary and Sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) essential oils. J Agric Food Chem, 2007; 55:7879-7885. [Web of Science] [Crossref]

34. Gedik H, Özkan YK. Cleaning Efficiency of Alkaline Peroxide Type Denture Cleansers on Silicone-Based Soft Lining Materials Colonized With Candida albicans. Balk J Stom, 2009; 13:35-40.

Citation Information: Balkan Journal of Dental Medicine. Volume 20, Issue 3, Pages 160–167, ISSN (Online) 2335-0245, DOI: https://doi.org/10.1515/bjdm-2016-0026, November 2016

Download (PDF, 327KB)

COMMENTS